Title
Dissecting the Rickettsia prowazekii genome: genetic and proteomic approaches
Document Type
Article
Publication Title
Annals of the New York Academy of Sciences
Abstract
The obligate nature of Rickettsia prowazekii intracellular growth places severe restrictions on the analysis of rickettsial gene function and gene expression. Fortunately, this situation is improving as methods for the genetic manipulation and proteomic analysis of this fascinating human pathogen become available. In this paper, we review the current status of rickettsial genetics and the isolation of rickettsial mutants using a genetic approach. In addition, the examination of rickettsial gene expression through characterization of the rickettsial proteome will be described. This will include a description of a high-throughput, accurate mass approach that has identified 596 rickettsial proteins in a complex rickettsial protein sample.
First Page
35
Last Page
46
DOI
10.1196/annals.1355.005
Publication Date
12-1-2005
Recommended Citation
Tucker, Aimee M.; Pannell, Lewis K.; and Wood, David O., "Dissecting the Rickettsia prowazekii genome: genetic and proteomic approaches" (2005). Department of Microbiology and Immunology Faculty and Staff Publications and Presentations. 39.
https://jagworks.southalabama.edu/med_microbio_immunology_faculty_staff_pubs/39