Title

Dissecting the Rickettsia prowazekii genome: genetic and proteomic approaches

Document Type

Article

Publication Title

Annals of the New York Academy of Sciences

Abstract

The obligate nature of Rickettsia prowazekii intracellular growth places severe restrictions on the analysis of rickettsial gene function and gene expression. Fortunately, this situation is improving as methods for the genetic manipulation and proteomic analysis of this fascinating human pathogen become available. In this paper, we review the current status of rickettsial genetics and the isolation of rickettsial mutants using a genetic approach. In addition, the examination of rickettsial gene expression through characterization of the rickettsial proteome will be described. This will include a description of a high-throughput, accurate mass approach that has identified 596 rickettsial proteins in a complex rickettsial protein sample.

First Page

35

Last Page

46

DOI

10.1196/annals.1355.005

Publication Date

12-1-2005

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