Honors Theses

Date of Award

5-2026

Document Type

Undergraduate Thesis

Degree Name

BS

Department

Biomedical Sciences

Faculty Mentor

Padmamalini Thulasiraman, Ph.D.

Advisor(s)

Sarah Urankar and Nancy Rice, Ph.D.

Abstract

Breast cancer is at the forefront of cancer deaths among women. Understanding the underlying mechanisms that drive its progression is the key to creating targeted therapies. A specific tyrosine kinase receptor, HER2, activated by heregulin (HRG), promotes breast cancer cell growth and development. This growth factor receptor is sialylated, and desialylation of HER2 causes autophosphorylation, which leads to activation of cellular signaling events. One of the oncogenes regulated by HER2 is fatty acid binding protein 5 (FABP5), which is well known to increase resistance to retinoic acid-mediated growth suppression. The objective of this study is to understand the role of Neu1 on the activation of HER2 and the regulation of the downstream target, FABP5. Our results have demonstrated that blocking the activity of sialidase Neuraminidase 1 (Neu1) with oseltamivir phosphate (OP) in breast cancer cell line MDA-MB-453 suppresses HRG-mediated activation of HER2 and its downstream targeted oncogene, FABP5. Furthermore, silencing of the Neu1 protein with siRNA suppressed the phosphorylation of HER2 and reduced the protein expression of FABP5. Knowledge of the interaction between Neu1 and HER2 could reveal novel medical therapies against these types of aggressive breast cancer, potentially improving the effectiveness of treatment and patient health overall.

Available for download on Saturday, July 07, 2029

Share

COinS