Honors Theses

Date of Award

11-2024

Document Type

Undergraduate Thesis

Degree Name

BS

Department

Biomedical Sciences

Faculty Mentor

Jason W. Coym

Advisor(s)

Christy Wheeler West, Robin Mockett

Abstract

High Performance Liquid Chromatography (HPLC) is an analytical chemist technique used to separate a sample into its components to characterize and study a specific molecule or compound. Traditionally, High-Performance Liquid Chromatography in different industries, such as the environmental and pharmaceutical fields, uses stationary phases that are already well characterized, such as the C18 stationary phase for separation. However, aromatic stationary phases have recently been produced, which have phenyl groups bonded to the interior surface of the column. Still, they have not been well characterized because of how modern they are. This project characterized several aromatic stationary phases (Biphenyl, pentafluorophenyl, and Phenyl-hexyl) so future separations can be more effectively performed. These aromatic stationary phases were also compared to a traditional C18 stationary phase. Selectivity tests showed that the aromatic stationary phases were more selective in certain conditions than in comparison to the C18 stationary phase. LSER Analysis that each aromatic stationary phase has slight differences in interactions that lead to the separation of solutes in a mixture. The results found throughout this project can be combined to show the underlying characteristics that yield the best separation for each of the three columns being studied, along with comparing to a more traditional and commonly used C18 stationary phase.

Comments

© 2024 Anastasia Davis ALL RIGHTS RESERVED

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