Digitized Honors Theses (2002-2017)

Date of Award

5-2013

Document Type

Thesis

Degree Name

BS

Department

Chemistry

Faculty Mentor

Alexandra Stenson

Abstract

This work describes the processes behind creating a method for extracting organic material from southeastern North American Indian pottery sherds, specifically those compounds used to identify remnants of yaupon tea known as Black Drink (caffeine, theobromine, and theophylline). The process for creating a Liquid Chromatography Mass Spectrometry (LC/MS) method to resolve the peaks of these compounds is also included, as well as several obstacles that arose during the experimental process. The overall goal of this experiment was to determine whether the sherds collected were involved in the brewing or storage of the beverage, Black Drink, and if additives were included in the brew to produce symptoms such as vomiting. Direct Analysis Real Time Mass Spectrometry (DART-MS) is also examined for its possible advantageous speed in screening sherds for the key compounds of interest. Some preliminary data are included, from a shard analysis that produced the proper mass peaks during DART-MS but resulted in a false positive once the LC/MS data were collected.

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