Document Type

Article

Publication Title

Journal of Cell Biology

Abstract

We report the identification and primary sequence of PCM-1, a 228-kD centrosomal protein that exhibits a distinct cell cycle-dependent association with the centrosome complex. Immunofluorescence microscopy using antibodies against recombinant PCM-1 demonstrated that PCM-1 is tightly associated with the centrosome complex through G1, S, and a portion of G2. However, late in G2, as cells prepare for mitosis, PCM-1 dissociates from the centrosome and then remains dispersed throughout the cell during mitosis before re-associating with the centrosomes in the G1 phase progeny cells. These results demonstrate that the pericentriolar material is a dynamic substance whose composition can fluctuate during the cell cycle.

First Page

783

Last Page

793

DOI

10.1083/jcb.124.5.783

Publication Date

1994

Department

College of Medicine

Comments

Copyright: © Balczon et al. All rights reserved.

Download

Share

COinS