Digitized Honors Theses (2002-2017)

Date of Award

5-2012

Document Type

Undergraduate Thesis

Degree Name

BS

Department

Biology

Faculty Mentor

Jason Coym, Ph.D.

Advisor(s)

Alexandra Stenson, Ph.D., Juan Mata, Ph.D.

Abstract

This work examines the effect of mobile phase composition on retention for various solutes using four different types of stationary phases: Cl 8, Cl 8 with dynamically added cholesterol, an Immobilized Artificial Membrane (IAM) phase, and a bonded cholesterol stationary phase. Through these experiments we wish to mimic the membrane of a cell and compare our results with pre-determined bioavailability values. Solutes were selected due to the differences in their retention with varying percentages of methanol in the mobile phase.

Chromatographic retention is plotted using the linear solvent strength model of log k vs. percent organic modifier, and extrapolated to estimate k at a I 00% water mobile phase. Values of these pure-water retention factors can then be correlated to various bioavailability metrics, as well as compared between our four chromatographic techniques. In addition, the linearity of these plots is examined and compared, via a comparison of the correlation coefficients of the solvent strength plots for a given solute on the four stationary phases.

Through these experiments we hope to get a better understanding of the behavior of cholesterol-coated stationary phases, and determine if these phases can be used as biomembrane mimics for measuring solute-membrane interactions. Of particular interest is the comparison between dynamically coated cholesterol and bonded cholesterol phases.

Download

Share

COinS